This section contains data from two studies. Differential susceptibility of human motor neurons to infection with Usutu and West Nile virus and Differential susceptibility of human motor neurons to infection with Usutu and West Nile virus.
Study to identify the susceptibility of human iPSC derived cortical and motor neurons to infection with USUV and WNV, with assessment of the underlying antiviral response.
Study to identify the mode of invasion across an in vitro human blood-brain barrier by Usutu and West Nile virus This study reports secondary objectives of a prospective observational study in adults hospitalized with an arbovirus-compatible disease syndrome in 21 hospitals in seven countries in southeast Europe over four arbovirus seasons (1 May 2016 - 31 October 2019). In this study, given the challenges in arbovirus diagnostics, we developed a diagnostic interpretation algorithm taking into account the (serological) outcomes and sample timing to study arbovirus prevalence in southeast Europe. We used enzyme-linked immunosorbent assay (ELISA) and custom-printed protein microarrays to simultaneously detect serum antibodies against Crimean-Congo haemorrhagic fever virus (CCHFV), tick-borne encephalitis virus (TBEV), Toscana virus (TOSV) and West Nile virus (WNV), followed by confirmation assays (virus neutralization test (VNT) or Indirect immunofluorescence test (IIFT)). Patients were sampled on day 0, 7, 28 and 60 post-hospital admission. This data encompasses the ELISA, protein microarray, VNT and IIFT data of CCHFV, TBEV, TOSV, WNV and USUV, for each included patient, country and year. Furthermore, it contains information about the sampling dates, estimated days post symptom onset (dpso) as well as its diagnostic interpretation according to the developed diagnostic interpretation algorithm.